Master's Program in Food Biotechnology
The course «Metabolic Engineering in Food Biotechnology» presents scientific knowledge on the practice of analysis and optimization of bioprocesses within cells to increase the cells' production of a certain substance. These processes are chemical networks that use a series of biochemical reactions and enzymes that allow cells to convert raw materials into molecules necessary for the cell’s survival. Metabolic engineering specifically seeks to mathematically model these networks, calculate a yield of useful products, and pin point parts of the network that constrain the production of these products. The ultimate goal of metabolic engineering is to be able to use these organisms to produce valuable substances on an industrial scale in a cost effective manner. Current examples include producing beer, wine, cheese, pharmaceuticals, and other biotechnology products.
In the course the students will learn basic principles of metabolism architecture, analysis of its network performance, mathematical description of the metabolic networks, regulatory principles in context of the metabolic network, effect of the environmental factors on their activity, inhibition and activation of the activity, additionally students will get practical skills how to analyse experimental data and interpret regression results. The designed program will prepare student for conducting independent research and practical activity in research laboratories and industrial plants.
Lectures. The PowerPoint slides of the lectures explicitly exploit modern educational technologies, such as: analysis of case studies, examples based on own experience, problem-based learning.
Lecture 1 – Introduction
Lecture 2 – The essences of metabolic engineering
Lecture 3 – Principle of metabolism
Lecture 4 – Review on cellular metabolism
Lecture 5 – Comprehensive models for cellular reactions
Lecture 6 – Growth kinetics and bioenergetics of cellular cultures
Lecture 7 – Fermentation processes
Lecture 8 – Fundamentals of balance-analysis in microbial research
Lecture 9 – Biochemical reaction network & MFA
Lecture 10 – Metabolic Control Analysis
Lecture 11 – Dynamic modeling of cellular reactions
Lecture 12 – HTS
Guidelines for Laboratory Training
Recommended textbooks
1. Cornish-Bowden, A. (2012). Fundamentals of enzyme kinetics, 4th ed. John Wiley & Sons.
2. Fell, D. (1997). Understanding the control of metabolism, London: Portland Press Ltd., 301 pages.
3. Stephanopoulos, G. N., Aristidou, A. A., and Nielsen, J. (1998) Metabolic engineering: Principles and methodologies, San Diego: Academic Press.
4. Viladsen, J., Nielsen, J., Liden, G. (2011) Bioreacton Engineering Principles, 3rd ed. Springer.
5. Dunn, I., Heinzle, E., Ingham, J., Prenosil, J.E. (2003) Biological Reaction Engineering. Wiley-VCH.
6. Klipp, E., Liebermeister, W., Wierling, C., Kowald, A., Lehrach, H., Herwig, R. (2009) Systems Biology. Wiley-VCH.
7. Sauro, H.M. (2014) Essentials of Biochemical modeling. Ambrosius Publishing.
8. Voit, E.O. (2000) Computational analysis of biochemical systems: A practical guide for biochemists and molecular biologists, Cambridge:Cambridge University Press, 531 pages.
Created / Updated: 21 April 2016 / 11 May 2016
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